Coding

Part:BBa_K3237011:Design

Designed by: Luke Saville, Kristi Turton   Group: iGEM19_Lethbridge   (2019-10-08)


NAB1


Assembly Compatibility:
  • 10
    COMPATIBLE WITH RFC[10]
  • 12
    COMPATIBLE WITH RFC[12]
  • 21
    INCOMPATIBLE WITH RFC[21]
    Illegal XhoI site found at 284
  • 23
    COMPATIBLE WITH RFC[23]
  • 25
    COMPATIBLE WITH RFC[25]
  • 1000
    COMPATIBLE WITH RFC[1000]


Design Notes

The NAB1 protein was codon optimized for use in C. reinhardtii .

Source

NAB1 sequence is based off a sequence attained from the NCBI database accession number EDP08495.1. The part was synthesized by IDT.

References

1. Mussgnug, J., Wobbe, L., Elles, I., Claus, C., Hamilton, M., Fink, A., Kahmann, U., Kapazoglou, A., Mullineaux, C., Hippler, M., Nickelsen, J., Nixon, P., and Kruse, O. (2005) NAB1 Is an RNA Binding Protein Involved in theLight-Regulated Differential Expression of theLight-Harvesting Antenna ofChlamydomonas reinhardtii. The plant cell. 17, 3409-3421

2. Beckmann, J., lehr, F., Finazzi, G., Kankamer, B., Posten, C., Wobbe, L., and Kruse, O. (2009) Improvement of light to biomass conversion by de-regulation of light-harvesting protein translation in Chlamydomonas reinhardtii. Journal of Biotechnology. 142, 70-77